Lopez A, Sims DE, Ablett RF, Skinner RE, Leger LW, Lariviere CM, Jamieson LA, Martinez-Burnes J, Zawadzka GG.
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
To determine the acute anti-inflammatory effects of topically applying emu oil.
96 male CD-l mice assigned randomly to 4 groups, each comprising 24 mice.
To induce auricular inflammation, 50 n: of a solution comprising 10 micro I of croton oil dissolved in 1 ml of acetone applied to the inner surface of the left auricle (pinna). One hour later, 3 or 5 I of emu oil (low- and high-dose groups, respectively) or 5 micro l of porcine (0 control) was applied to the left pinna. Control mice remained untreated. Six I per group were euthanatized 3, 6,12, and 24 hours after induction of inflamn Specimens of auricular tissue (ear plugs) were obtained, using a 6-mm biops punch. Magnitude of swelling was calculated as the weight difference between (inflamed) and right (noninflamed) ear plugs; degree of edema was determined by the difference between wet and dry weights of the left ear plug.
Magnitude of swelling was significantly reduced at 6 and 12 hours in mice tr with emu or porcine oil, compared with controls. The greatest reduction was detected in the high-dose emu group at 6 hours. Compared with controls degree of edema was significantly reduced at 6 hours only in the high-dose g whereas by 12 hours, all groups treated with oils had significantly less edema controls. At 24 hours, magnitude of swelling and degree of edema did not differ among groups.
Topically applied emu oil significantly reduce severity of acute auricular inflammation induced by croton oil in mice.